Journal: Cell
Article Title: Commensal Microbiota Promote Lung Cancer Development via γδ T Cells
doi: 10.1016/j.cell.2018.12.040
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies In vivo mAb UC7–13D5 BioXCell UC7–13D5 Cat# BE0070 In vivo mAb IL-17A BioXCell 17F3 Cat# BE0173 In vivo mAb GL3 Dr. O’Brien (National Jewish Health, Denver) FC: CD45 BD Bioscience 30-F11 Cat#562420 FC: CD16/CD32 BD Bioscience 2.4G2 Cat#553142 FC: CD45.2 Biolegend 104 Cat#109841 FC: CD45.1 Biolegend A20 Cat#110743 FC: Ly6G Biolegend 1A8 Cat#127622 FC: CD11b eBioscience M1/70 Cat# 101243 FC: TCRβ Biolegend H57 −597 Cat# 109220 FC: TCRγδ Biolegend GL3 Cat# 118118 FC: TCRγδ Biolegend UC7–13D5 Cat# 107504 FC: CD3 Biolegend 17A2 Cat# 100228 FC: CD4 Biolegend RM4–5 Cat# 100552 FC: CD8 BD Bioscience 53–6.7 Cat# 563786 FC: CD11c eBioscience N418 Cat# 25–0114–82 FC: CD103 Biolegend 2E7 Cat# 121414 FC: Ly6C Biolegend HK1.4 Cat# 128014 FC: Vγ1 (Vg1.1) Biolegend 2.11 Cat# 141104 FC: Vγ4 (Vγ2) Biolegend UC3–10A6 Cat# 137706 FC: IFN-γ BD Bioscience XMG1.2 Cat# 554412 FC: IL-17A Biolegend TC11–18H10.1 Cat# 506916 FC: TNFa eBioscience MP6-XT22 Cat# 11–7321–82 FC: IL-17F Biolegend 9D3.1C8 Cat# 517008 FC: CD44 Biolegend IM7 Cat# 103039 FC: CD27 eBioscience LG.3A10 Cat# 11–0271–85 FC: CD69 Biolegend H1.2F3 Cat# 104527 FC: PD-1 Biolegend RMP1–30 Cat# 109110 FC: T-bet eBioscience eBio4B10 Cat# 12–5825–82 FC: RORγt eBioscience B2D Cat# 17–6981–82 FC: PLZF eBioscience Mags.21F7 Cat# 12–9320–82 FC: Ki67 BD Bioscience B56 Cat# 561277 FC: 17D1 Dr. Robert Tigelaar and Julia Lewis (Yale University, CT) 17D1 IHC: Purified Rat Anti-Mouse Ly-6G BD Pharmingen 1A8 Cat# 551459 IHC: Mouse mAb against human TCRδ Santa Cruz H-41 Cat# sc-100289 IHC: Ki-67 (D3B5) Rabbit mAb (Mouse Preferred) Cell Signaling #12202 Bacterial and Virus Strains Adenovirus ( Sftpc-Cre ) Viral Vector Core, University of Iowa Ad5mSPC-Cre Chemicals, Peptides, and Recombinant Proteins Recombinant mouse IL-1β R&D Systems 401-ML-005 Recombinant mouse IL-23 R&D Systems 1887-ML-010/CF Recombinant mouse IL-22 R&D Systems 582-ML-010 Recombinant human amphiregulin Sigma A7080 Lipopolysaccharide from E. coli O111:B4 (LPS) Invivogen tlrl-eblps Peptidoglycan from S. aureus (PGN) Invivogen tlrl-pgns2 Critical Commercial Assays Mouse IL-17A (homodimer) ELISA eBioscience 88–7371–86 CellTiter-Glo® Luminescent Cell Viability Assay Promega G7571 Deposited Data RNA-seq GEO {"type":"entrez-geo","attrs":{"text":"GSE114340","term_id":"114340"}} GSE114340 16S sequencing SRA PRJNA492954 Experimental Models: Organisms/Strains B6 CD45.2: C57BL/6J Jackson Laboratory 000664 Myd88 Null: B6.129P2(SJL)- Myd88 tm1.1Defr /J Jackson Laboratory 009088 B6J.129(B6N)- Gt(ROSA)26Sor tm1(CAG-cas9*,-EGFP)Fezh /J Jackson Laboratory, crossed to KP mice 026175 B6 CD45.1: B6.SJL- Ptprc a Pepc b /BoyJ Jackson Laboratory, crossed to KP mice 002014 Open in a separate window KEY RESOURCES TABLE
Techniques: In Vivo, Purification, Virus, Plasmid Preparation, Recombinant, Enzyme-linked Immunosorbent Assay, Cell Viability Assay, Sequencing
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